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Image Search Results
Journal: PLoS ONE
Article Title: (2-Hydroxypropyl)-β-Cyclodextrin Is a New Angiogenic Molecule for Therapeutic Angiogenesis
doi: 10.1371/journal.pone.0125323
Figure Lengend Snippet: (A) Ischemic/non-ischemic limb blood flow ratio assessed by LDBF imager in mice that received different concentrations of 2HP-β-CD for 28 days. Data represent the mean ± S.E.M. ( n = 8 mice per group). ** p < 0.01 and *** p < 0.001 versus the PBS-treated mice. (B) 2HP-β-CD-stimulated angiogenesis and arteriogenesis in C57BL/6 mice. EC marker CD31 (red) and SMC marker αSMA (green) were detected by double immunofluorescence in the ischemic hindlimb of PBS-treated (upper panel) and 2HP-β-CD-treated (lower panel) mice. Representative immunofluorescence images of each marker and merged images are shown. (C) CD31 positive microvessels, αSMA positive vessels and αSMA positive vascular area were quantified using Image J software. Data represent the mean ± S.E.M. ( n = 4 mice per group). * p < 0.05 versus the PBS-treated control mice. Scale bar = 50 μm.
Article Snippet: Blood flow in the ischemic hindlimb was measured using a
Techniques: Marker, Immunofluorescence, Software, Control
Journal: PLoS ONE
Article Title: (2-Hydroxypropyl)-β-Cyclodextrin Is a New Angiogenic Molecule for Therapeutic Angiogenesis
doi: 10.1371/journal.pone.0125323
Figure Lengend Snippet: A, Ischemic/non-ischemic limb blood flow ratio assessed by LDBF imager in mice in 4 experimental groups; PBS-treated mice with or without L-NAME, 2HP-β-CD-treated mice with or without L-NAME. Data represent the mean ± S.E.M. (n = 10 mice per group). * p < 0.05, 2HP-β-CD-treated mice versus PBS-treated mice. § p < 0.05, 2HP-β-CD-treated mice versus 2HP-β-CD with L-NAME-treated mice; # p < 0.05, PBS with L-NAME-treated mice versus 2HP-β-CD with L-NAME-treated mice. B, Immunohistochemical analysis of CD31 in ischemic hindlimbs of PBS-treated and 2HP-β-CD-treated mice with or without L-NAME. Representative images (upper panel) from calf muscle on postoperative day 28 are shown. Quantitative analysis of CD31-positive microvessel density is also shown (lower panel). Data represent the mean ± S.E.M. (n = 5 mice per group). * p < 0.05. Scale bar = 100 μm.
Article Snippet: Blood flow in the ischemic hindlimb was measured using a
Techniques: Immunohistochemical staining